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Document ID (ISN)68912
CIS number 97-495
ISSN - Serial title 1250-3274 - Archives des maladies professionnelles et de médecine du travail
Year 1996
Convention or series no.
Author(s) Lehucher-Michel M.P., Ait Amara Y., Botta A.
Title Micronucleus assay on exfoliated urothelial cells: Effect of smoking and application to occupational biomonitoring
Original title Application du test de numération des micronoyaux aux cellules urothéliales exfoliées: effet du tabagisme et intérêt en médecine du travail [in French]
Bibliographic information Oct. 1996, Vol.57, No.6, p.429-437. Illus. 82 ref.
Abstract Exposure to genotoxic chemicals increases the incidence of bladder cancer. Smoking seems to have an additive effect on this cancer risk. Exfoliated bladder cell micronuclei assays may constitute a useful testing for genotoxic effects in populations with an increased risk of bladder cancer due to exposure to chemical substances. A micronucleus assay was applied to 73 healthy donors not occupationally exposed to genotoxic chemicals. Among the variables studied in 50 subjects presenting a number of analyzed urothelial cells at least equal to 1000, only smoking showed a significant effect on micronucleated cell rates (P = 0.007). Micronucleated cell levels in ex-smokers were slightly lower but not significantly different from that of smokers suggesting that smoking could generate clones of basal micronucleated cells in urothelial tissues. The importance of extending the micronucleus assay to target tissues is stressed.
Descriptors (primary) mutagenic effects; smoking; occupational medicine; genetic effects; exposure tests; exposure evaluation
Descriptors (secondary) evaluation of technique
Document type D - Periodical articles
Country / State or ProvinceFrance
Subject(s) Medicine, hygiene and first aid
Broad subject area(s) Occupational medicine, epidemiology
Browse category(ies) Antifertility and prenatal effects
Genetic factors in reaction to exposures
Exposure evaluation
Biological monitoring