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CISDOC database

Document ID (ISN)56235
CIS number 91-1201
ISSN - Serial title 0355-3140 - Scandinavian Journal of Work, Environment and Health
Year 1990
Convention or series no.
Author(s) Wass U., Belin L.
Title An in vitro method for predicting sensitizing properties of inhaled chemicals
Bibliographic information June 1990, Vol.16, No.3, p.208-214. Illus. 63 ref.
Abstract A method for monitoring chemical reactivity in aqueous solutions, at neutral pH and 37░C, was developed. The chemical was allowed to react with a lysine-containing peptide, and the reaction was monitored with high-performance liquid chromatography. Simple acids, bases, and solvents did not react with the peptide, whereas isocyanates, anhydrides, and chloramine-T, substances well known for their sensitising and asthma-inducing properties, did. Thus a positive test strongly suggested that the chemical had the potential to act as a hapten and cause sensitisation when inhaled. Prepolymers of diphenylmethane diisocyanate were considerably more reactive than prepolymers of toluene diisocyanate or hexamethylene diisocyanate. Isocyanates blocked with caprolactam, butanone oxime, malonic acid diethylester, or isononyl phenol showed no reactivity. This result suggested a significantly reduced risk of respiratory reactions when such blocked isocyanates are handled at room temperature.
Descriptors (primary) isocyanates; inhalation; sensitization; experimental pathology; chemical products
Descriptors (secondary) high performance chromatography; allergic asthma; allergic reactivity; biochemical tests; in vitro experiments
Document type D - Periodical articles
Country / State or ProvinceSweden
Subject(s) Toxic and dangerous substances
Broad subject area(s) Chemical safety
Browse category(ies) Isocyanates
Cyano compounds