| Document ID (ISN) | 47007 |
| CIS number |
86-1862 |
| ISSN - Serial title |
0096-1736 - Journal of Occupational Medicine |
| Year |
1986 |
| Convention or series no. |
|
| Author(s) |
Skipper P.L., Bryant M.S., Tannenbaum S.R., Groopman J.D. |
| Title |
Analytical methods for assessing exposure to 4-aminobiphenyl based on protein adduct formation |
| Bibliographic information |
Aug. 1986, Vol.28, No.8, p.643-646. Illus. 8 ref. |
| Abstract |
Past studies with animals have demonstrated that 4-aminobiphenyl (ABP) administration results in the formation of appreciable amounts of adducts between the carcinogen and both serum albumin and haemoglobin. The haemoglobin adduct is relatively stable in vivo, but may be readily hydrolysed in vitro to regenerate ABP. The formation of this adduct reflects a mixed-function-oxidase-mediated metabolic pathway operating directly on the amine. The predominant albumin adduct, 3-(tryptophan-N1-yl)-4-acetylaminobiphenyl, reflects the contribution of N-acetyltransferase activity as well as mixed-function-oxidase activity to the overall metabolism. The simultaneous measurement of these 2 different adducts thus offers an opportunity to investigate the role of both ABP and acetylator phenotype in bladder carcinogenesis. An analytical method, using gas chromatography coupled with electron capture detection, was developed to quantitate ABP adducted to haemoglobin. |
| Descriptors (primary) |
4-aminobiphenyl; exposure tests; determination in blood; bladder tumour |
| Descriptors (secondary) |
description of technique |
| Document type |
D - Periodical articles |
| Subject(s) |
Toxic and dangerous substances
|
| Broad subject area(s) |
|
| Browse category(ies) |
Aromatic amines
Biological monitoring
Diseases of the kidney and the urinary system
|
