|Document ID (ISN)||46983|
|ISSN - Serial title
||0387-821X - Journal of UOEH
|Convention or series no.
||Koga M., Hori H., Tanaka I., Akiyama T., Inoue N.
||Quantitative analysis of urinary ethylene glycol in rats
||Mar. 1985, Vol.7, No.1, p.45-49. Illus. 7 ref.
||Ethylene glycol in urine was separated by azeotropic distillation and esterified with n-butylboronic acid. The derivatised ethylene glycol was separated by gaz chromatography at 70° on a 2m by 3mm glass column of 2% OV-225 on 60-80 mesh Uniport HPS. A flame ionisation detector was used. Known amounts of z-methylcyclohexanone were included in the samples as internal standards. A linear calibration curve was obtained up to 500µg/mL of ethylene glycol. The detection limit was 10µg/mL and the relative standard deviation was 3.5% for 100µg/mL of ethylene glycol. This method was used to determine the urinary excretion of ethylene glycol in rats exposed to ethylene oxide at various concentrations (50-500ppm). The amounts of ethylene glycol excreted were sigmoidally dependent on the concentration of ethylene oxide to which the rats had been exposed.
||ethylene glycol; ethylene oxide; gas-liquid chromatography; determination in urine; exposure evaluation
||description of technique; animal experiments
||D - Periodical articles
||Toxic and dangerous substances
|Broad subject area(s)
||Occupational medicine, epidemiology