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Document ID (ISN)46983
CIS number 86-1646
ISSN - Serial title 0387-821X - Journal of UOEH
Year 1985
Convention or series no.
Author(s) Koga M., Hori H., Tanaka I., Akiyama T., Inoue N.
Title Quantitative analysis of urinary ethylene glycol in rats
Bibliographic information Mar. 1985, Vol.7, No.1, p.45-49. Illus. 7 ref.
Abstract Ethylene glycol in urine was separated by azeotropic distillation and esterified with n-butylboronic acid. The derivatised ethylene glycol was separated by gaz chromatography at 70° on a 2m by 3mm glass column of 2% OV-225 on 60-80 mesh Uniport HPS. A flame ionisation detector was used. Known amounts of z-methylcyclohexanone were included in the samples as internal standards. A linear calibration curve was obtained up to 500µg/mL of ethylene glycol. The detection limit was 10µg/mL and the relative standard deviation was 3.5% for 100µg/mL of ethylene glycol. This method was used to determine the urinary excretion of ethylene glycol in rats exposed to ethylene oxide at various concentrations (50-500ppm). The amounts of ethylene glycol excreted were sigmoidally dependent on the concentration of ethylene oxide to which the rats had been exposed.
Descriptors (primary) ethylene glycol; ethylene oxide; gas-liquid chromatography; determination in urine; exposure evaluation
Descriptors (secondary) description of technique; animal experiments
Document type D - Periodical articles
Subject(s) Toxic and dangerous substances
Broad subject area(s) Occupational medicine, epidemiology
Browse category(ies) Alcohols
Ethylene oxide
Exposure evaluation
Glycols
Ethers