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Document ID (ISN)34977
CIS number 81-185
ISSN - Serial title 0032-6291 - Pracovní lékařství
Year 1980
Convention or series no.
Author(s) Nerudová J., Kopecký J., Gut I., Holeček V.
Title In vitro investigation of acrylonitrile metabolism in the rat
Original title Metabolismus akrylonitrilu u krysy in vitro [in Czech]
Bibliographic information Mar. 1980, Vol.32, No.3, p.98-103. Illus. 20 ref.
Abstract Research on the conversion of acrylonitrile (AN) into cyanide by liver microsomes. The dependance of conversion on the concentration of cofactors, on the concentration of microsomal protein, and pH of the mixture during incubation indicate the role of liver monoxygenases in conversion. Addition of an inhibitor of the epoxy hydratase enzyme significantly decreases conversion of AN to cyanide, this supports the hypothesis according to which the first stage of AN biotransformation is AN oxidation to glycidonitrile and a subsequent hydration of this nitrile into glycolaldehyde cyanohydrin. The presence of cyanohydrin in the incubation mixture seems to be established by processing of this mixture at pH 6.3 after incubation, resulting in the cyanohydrin - cyanide balance being shifted in favour of cyanide, and in a 3-fold increase in the conversion rate of AN to cyanide.
Descriptors (primary) metabolic process; toxicology; acrylonitrile
Descriptors (secondary) in vitro experiments; animal experiments
Document type D - Periodical articles
Subject(s) Occupational pathology
Broad subject area(s)
Browse category(ies) Cyano compounds