Document ID (ISN) | 34977 |
CIS number |
81-185 |
ISSN - Serial title |
0032-6291 - Pracovní lékařství |
Year |
1980 |
Convention or series no. |
|
Author(s) |
Nerudová J., Kopecký J., Gut I., Holeček V. |
Title |
In vitro investigation of acrylonitrile metabolism in the rat |
Original title |
Metabolismus akrylonitrilu u krysy in vitro [in Czech] |
Bibliographic information |
Mar. 1980, Vol.32, No.3, p.98-103. Illus. 20 ref. |
Abstract |
Research on the conversion of acrylonitrile (AN) into cyanide by liver microsomes. The dependance of conversion on the concentration of cofactors, on the concentration of microsomal protein, and pH of the mixture during incubation indicate the role of liver monoxygenases in conversion. Addition of an inhibitor of the epoxy hydratase enzyme significantly decreases conversion of AN to cyanide, this supports the hypothesis according to which the first stage of AN biotransformation is AN oxidation to glycidonitrile and a subsequent hydration of this nitrile into glycolaldehyde cyanohydrin. The presence of cyanohydrin in the incubation mixture seems to be established by processing of this mixture at pH 6.3 after incubation, resulting in the cyanohydrin - cyanide balance being shifted in favour of cyanide, and in a 3-fold increase in the conversion rate of AN to cyanide. |
Descriptors (primary) |
metabolic process; toxicology; acrylonitrile |
Descriptors (secondary) |
in vitro experiments; animal experiments |
Document type |
D - Periodical articles |
Subject(s) |
Occupational pathology
|
Broad subject area(s) |
|
Browse category(ies) |
Cyano compounds
|