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CISDOC database

Document ID (ISN)33126
CIS number 80-435
ISSN - Serial title 0003-0805 - American Review of Respiratory Disease
Year 1979
Convention or series no.
Author(s) Tse C.S.T., Chen S.E., Bernstein I.L.
Title Induction of murine reaginic antibodies by toluene diisocyanate - An animal model of immediate hypersensitivity reactions to isocyanates.
Bibliographic information Oct. 1979, Vol.120, No.4, p.829-835. 17 ref.
Abstract Investigation to establish an analogous homocytotropic antibody model in mice immunised with isocyanate-protein conjugates. Tolylene diisocyanate (TDI) was conjugated to serum albumins; the amount of ligand was determined spectrophotometrically. Mice were immunised and boosted 28 days later with 100µg TDI conjugated proteins or unconjugated proteins plus 1mg alum. Sera were assessed for 48h passive cutaneous anaphylaxis; sera from mice immunised with conjugated serum albumin demonstrated passive cutaneous anaphylaxis titers of 1:57 or higher when challenged with homologous conjugates and somewhat lower titers after reactions with heterologous conjugates. Mice immunised with carrier proteins alone produced homocytotropic antibody (1:40) to corresponding unconjugated proteins and homologous TDI-conjugates (1:5 to 1:10) but not to heterologous carrier proteins or TDI-conjugates. Findings are shown in tabular form.
Descriptors (primary) tolylene diisocyanate; hypersensitivity; immunology
Descriptors (secondary) 2,4-tolylene diisocyanate; immunization; animal experiments; serum protein changes
Document type -
Subject(s) Toxic and dangerous substances
Broad subject area(s)
Browse category(ies) Isocyanates
Cyano compounds