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Document ID (ISN)112413
CIS number 12-0170
ISSN - Serial title 1076-2752 - Journal of Occupational and Environmental Medicine
Year 2011
Convention or series no.
Author(s) Gambelunghe A., Buratta S., Ferrara G., Mozzi R., Marchetti C., Murgia N., Muzi G.
Title Phosphatidylserine metabolism in human lymphoblastic cells exposed to chromium (IV)
Bibliographic information July 2011, Vol.53, No.7, p.776-781. Illus. 27 ref.
Internet access Phosphatidylserine_metabolism_[BUY_THIS_ARTICLE] [in English]
ILO_LABORDOC_[INTRANET_ACCESS] [in English]
Abstract Hexavalent chromium compounds are widely found in working environments. These compounds can cause apoptosis in human cells, but the mechanisms underlying chromium-induced apoptosis are not clear. A marker of apoptosis is the exposure of phosphatidylserine on cell membrane and the modification of phosphatidylserine metabolism. The aim of this study was to verify whether chromium could cause phosphatidylserine exposure and modification of its metabolism in human lymphoblastic leukemia cell line (MOLT-4). Phosphatidylserine exposure was evaluated by annexin V binding whereas phosphatidylserine metabolism was studied measuring the incorporation of [3H]serine. Cell treatment with hexavalent chromium increases phosphatidylserine exposure and cell apoptosis, but decreases the incorporation of [3H]serine into phosphatidylserine in a dose- and time-dependent manner.
Descriptors (primary) chromium and compounds; leukaemia; lymphocytes; metabolic studies
Descriptors (secondary) radioactive substances; cell culture; immunology; in vitro experiments; dose-response relationship
Document type D - Periodical articles
Subject(s) Toxic and dangerous substances
Occupational pathology
Broad subject area(s) Occupational medicine, epidemiology
Chemical safety
Browse category(ies) Toxicology