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Document ID (ISN)111347
CIS number 11-0042
ISSN - Serial title 1745-6673 - Journal of Occupational Medicine and Toxicology
Year 2010
Convention or series no.
Author(s) Gasnier C., Benachour N., Clair E., Travert C., Langlois F., Laurant C., Decroix-Laporte C., SÚralini G.E.
Title Dig1 protects against cell death provoked by glyphosate-based herbicides in human liver cell lines
Bibliographic information 2010, 5:28, 13p. Illus. 3 ref.
Internet access Dig1_protects_against_cell_death.pdf [in English]
Abstract Glyphosate-based herbicides can provoke in vivo toxicity and in human cells. These herbicides are commonly found in the environment, surface waters and as food residues of glyphosate-tolerant genetically modified plants. In order to better understand their effects on cells from liver, a major detoxification organ, this study investigates their mechanism of action and possible protection by medicinal plant extracts called Dig1. The cytotoxicity pathways of four formulations of glyphosate-based herbicides were studied using human hepatic cell lines HepG2 and Hep3B, known models to study xenobiotic effects. Mitochondrial succinate dehydrogenase activity and caspases 3/7 were monitored for cell mortality and protection by Dig1, as well as cytochromes P450 1A1, 1A2, 3A4 and 2C9 and glutathione-S-transferase to approach the mechanism of actions. All the four glyphosate formulations were found to provoke liver cell death, with adjuvants having stronger effects than glyphosate alone. Glyphosate is able to provoke intracellular disruption in hepatic cell lines at different levels, but a mixture of medicinal plant extracts Dig1 can to some extent protect human cell lines against these pollutants.
Descriptors (primary) glyphosate; medicinal plants; cell culture; medical prevention and treatment; cytotoxic effects
Descriptors (secondary) enzyme activity; in vitro experiments
Document type D - Periodical articles
Subject(s) Toxic and dangerous substances
Occupational pathology
Broad subject area(s) Occupational medicine, epidemiology
Chemical safety
Browse category(ies) Agricultural chemicals
Toxicology