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Document ID (ISN)110563
CIS number 10-0137
ISBN(s) 978-2-89631-337-2
Year 2009
Convention or series no. Etudes et recherches Rapport RF-595
Author(s) Duchaine C., Létourneau V., Nehmé B., Mériaux A., Gilbert Y., Veillette M.
Title Characterization of bioaerosols by molecular microbial ecology
Original title Caractérisation des bioaérosols par écologie microbienne moléculaire [in French]
Bibliographic information Institut de recherche Robert-Sauvé en santé et en sécurité du travail du Québec (IRSST), 505 boul. de Maisonneuve Ouest, Montreal (Quebec) H3A 3C2, Canada, 2009. viii, 37p. Illus. 35 ref. Price: CAD 6.30. Downloadable version (PDF format) free of charge.
Internet access [in French]
Abstract Pigsty workers are exposed to organic contaminants and gases. Most studies on the workplace atmosphere in pigsties have so far been based solely on the culture of microorganisms, which limits the microbial biodiversity and underestimates the microbiological load. Other methods, based on molecular biology, have been used to analyze the microbiology of soils, but not that of bioaerosols. The researchers applied these two methods for comparison purposes and studied the impact of seasons and the manure management method on the type and load of the bioaerosols in pig operations. They show the usefulness of molecular biology tools in characterizing bioaerosols in an agricultural environment, but conclude that the analyses based on this method also have limitations that could have an impact on the extent of the biodiversity observed. As a result, classical culture methods for microorganisms remain an essential complement.
Descriptors (primary) Quebec; swine; bacteria; aerosols; exposure evaluation; livestock rearing
Descriptors (secondary) Canada; sampling and analysis; reliability; report; description of technique
Document type E - Books, reports, proceedings
Subject(s) Toxic and dangerous substances
Broad subject area(s) Biological hazards
Browse category(ies) Exposure evaluation
Biological hazards