|Document ID (ISN)||109993|
|ISSN - Serial title
||1059-924X - Journal of Agromedicine
|Convention or series no.
||Kisby G.E., Muniz J.F., Scherer J., Lasarev M.R., Koshy M., Kow Y.W., McCauley L.
||Oxidative stress and DNA damage in agricultural workers
||2nd Quarter 2009, Vol.14, No.2, p.206-214. Illus. 22 ref.
||The objective of this study was to examine the relationship between organophosphate (OP) pesticide exposure and oxidative stress and DNA damage. Urine samples of pesticide applicators and farm workers working in the fruit orchards of Oregon were analysed for OP metabolites and 8-hydroxy-2'-deoxyguanosine (8-OH-dG). Lymphocytes were analyzed for oxidative DNA repair activity and DNA damage (Comet assay), and was serum analysed for lipid peroxides. Cellular DNA damage was validated using lymphocyte cell cultures. Compared to unexposed populations, urinary OP metabolites were significantly higher in farm workers and applicators, with 8-OH-dG levels being 8.5 and 2.3 times higher, respectively and malondialdehyde levels 4.9 and 24 times higher, respectively. DNA damage and oxidative DNA repair were also significantly greater in lymphocytes of applicators and farm workers.
||Oregon; insecticides; chromosome changes; pesticide spraying; agriculture; orchards; risk factors
||lipid peroxides; lymphocytes; USA; determination in urine; DNA; determination in blood; organophosphorus compounds
||D - Periodical articles
|Country / State or Province||USA|
||Agriculture, fishing, animal husbandry
|Broad subject area(s)
||Occupational medicine, epidemiology
||Antifertility and prenatal effects
Genetic factors in reaction to exposures