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Document ID (ISN)109993
CIS number 09-811
ISSN - Serial title 1059-924X - Journal of Agromedicine
Year 2009
Convention or series no.
Author(s) Kisby G.E., Muniz J.F., Scherer J., Lasarev M.R., Koshy M., Kow Y.W., McCauley L.
Title Oxidative stress and DNA damage in agricultural workers
Bibliographic information 2nd Quarter 2009, Vol.14, No.2, p.206-214. Illus. 22 ref.
Abstract The objective of this study was to examine the relationship between organophosphate (OP) pesticide exposure and oxidative stress and DNA damage. Urine samples of pesticide applicators and farm workers working in the fruit orchards of Oregon were analysed for OP metabolites and 8-hydroxy-2'-deoxyguanosine (8-OH-dG). Lymphocytes were analyzed for oxidative DNA repair activity and DNA damage (Comet assay), and was serum analysed for lipid peroxides. Cellular DNA damage was validated using lymphocyte cell cultures. Compared to unexposed populations, urinary OP metabolites were significantly higher in farm workers and applicators, with 8-OH-dG levels being 8.5 and 2.3 times higher, respectively and malondialdehyde levels 4.9 and 24 times higher, respectively. DNA damage and oxidative DNA repair were also significantly greater in lymphocytes of applicators and farm workers.
Descriptors (primary) Oregon; insecticides; chromosome changes; pesticide spraying; agriculture; orchards; risk factors
Descriptors (secondary) lipid peroxides; lymphocytes; USA; determination in urine; DNA; determination in blood; organophosphorus compounds
Document type D - Periodical articles
Country / State or ProvinceUSA
Subject(s) Agriculture, fishing, animal husbandry
Broad subject area(s) Occupational medicine, epidemiology
Browse category(ies) Antifertility and prenatal effects
Agricultural chemicals
Genetic factors in reaction to exposures