| Document ID (ISN) | 104942 |
| CIS number |
06-447 |
| ISSN - Serial title |
1545-9624 - Journal of Occupational and Environmental Hygiene |
| Year |
2005 |
| Convention or series no. |
|
| Author(s) |
Webster A.R., Lee J.Y., Deininger R.A. |
| Title |
Rapid assessment of microbial hazards in metalworking fluids |
| Bibliographic information |
Apr. 2005, Vol.2, No.4, p.213-218. Illus. 20 ref. |
| Abstract |
In this study, a filtration-based rapid adenosine triphosphate (ATP) bioluminescence assay, which takes less than 10min to perform, was evaluated as a test method for estimating total number of bacteria in metalworking fluid. This evaluation used two types of metalworking fluid (soluble and semi-synthetic) that were inoculated using Pseudomonas aeruginosa and spoiled metalworking fluid. Daily parallel testing was completed using the rapid ATP assay and the standard plate count methods. Test results were evaluated by statistical correlation and regression procedures for each fluid type. Study results indicate that the rapid ATP assay is strongly correlated to the standard plate count method for soluble and semi-synthetic fluids. |
| Descriptors (primary) |
bacteria; cutting fluids; sampling and analysis; biological hazards; metalworking industry |
| Descriptors (secondary) |
adenosine triphosphate; description of technique; evaluation of technique; Pseudomonas |
| Document type |
D - Periodical articles |
| Country / State or Province | USA |
| Subject(s) |
Metal production and metalworking
|
| Broad subject area(s) |
Biological hazards
|
| Browse category(ies) |
Biological hazards
Metalworking industry
|
