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Document ID (ISN)103293
CIS number 04-466
ISSN - Serial title 0003-4878 - Annals of Occupational Hygiene
Year 2003
Convention or series no.
Author(s) Thorne P.S., Bartlett K.H., Phipps J., Kulhankova K.
Title Evaluation of five extraction protocols for quantification of endotoxin in metalworking fluid aerosol
Bibliographic information Jan. 2003, Vol.47, No.1, p.31-36. Illus. 29 ref.
Abstract Occupational exposures to endotoxin-contaminated, water-based metalworking fluids (MWFs) are thought to contribute to cases of respiratory illness. The method most commonly used to quantify endotoxin is the Limulus amebocyte lysate (LAL) assay and this is the basis for the ASTM method E2144-01. In this study, multiple samples with similar mass and endotoxin loading were generated in order to compare four alternative extraction methods with the ASTM method. Aerosols with three concentrations of endotoxins (4.5, 350 and 1141EU/m3) were collected simultaneously on multiple filter samples using an exposure chamber system that provides a uniform distribution of MWF mist. It was found that the ASTM method yielded comparable estimations of MWF endotoxin aerosol concentrations but with higher variability than the four other extraction methods. In particular, extraction into pyrogen-free water at 25░C was more precise and simpler than the ASTM method.
Descriptors (primary) bacterial toxins; aerosols; biological hazards; exposure evaluation; metalworking industry; cutting fluids
Descriptors (secondary) reliability; comparative study; description of technique; sampling and analysis
Document type D - Periodical articles
Country / State or ProvinceUSA
Subject(s) Metal production and metalworking
Broad subject area(s) Biological hazards
Browse category(ies) Exposure evaluation
Metalworking industry
Biological hazards