|Document ID (ISN)||103293|
|ISSN - Serial title
||0003-4878 - Annals of Occupational Hygiene
|Convention or series no.
||Thorne P.S., Bartlett K.H., Phipps J., Kulhankova K.
||Evaluation of five extraction protocols for quantification of endotoxin in metalworking fluid aerosol
||Jan. 2003, Vol.47, No.1, p.31-36. Illus. 29 ref.
||Occupational exposures to endotoxin-contaminated, water-based metalworking fluids (MWFs) are thought to contribute to cases of respiratory illness. The method most commonly used to quantify endotoxin is the Limulus amebocyte lysate (LAL) assay and this is the basis for the ASTM method E2144-01. In this study, multiple samples with similar mass and endotoxin loading were generated in order to compare four alternative extraction methods with the ASTM method. Aerosols with three concentrations of endotoxins (4.5, 350 and 1141EU/m3) were collected simultaneously on multiple filter samples using an exposure chamber system that provides a uniform distribution of MWF mist. It was found that the ASTM method yielded comparable estimations of MWF endotoxin aerosol concentrations but with higher variability than the four other extraction methods. In particular, extraction into pyrogen-free water at 25░C was more precise and simpler than the ASTM method.
||bacterial toxins; aerosols; biological hazards; exposure evaluation; metalworking industry; cutting fluids
||reliability; comparative study; description of technique; sampling and analysis
||D - Periodical articles
|Country / State or Province||USA|
||Metal production and metalworking
|Broad subject area(s)