ILO Home
Go to the home page
Site map | Contact us Français | Español

CISDOC database

Document ID (ISN)101601
CIS number 03-792
ISSN - Serial title 0376-7639 - Médecine du travail & Ergonomie / Arbeidsgezondheitszorg & Ergonomie
Year 2000
Convention or series no.
Author(s) Beyst H., de Pauw P., de Thibault de Boesinghe L., Min Jou W., Segers P.
Title Implementing safety rules for working with ethidium bromide in laboratories
Original title Implementatie van veiligheidsvoorschriften bij het gebruik van ethidiumbromide in laboratoria [in Dutch]
Bibliographic information 2000, Vol.XXXVII, No.1, p.31-37. Illus. 4 ref.
Abstract Ethidium bromide (EtBr) is a strong mutagenic agent, frequently used in molecular biology. A number of measurements and laboratory visits led to the conclusion that the complex ways in which this substance is handled poses various safety and health problems. To detect EtBr, the use of an UV hand lamp is necessary. Detection depends on a minimal concentration of EtBr (approximately 1µg/mL) and the nature of the materials contaminated by EtBr. Taking these measurements, experiences and interviews with laboratory personnel into account, various prevention measures are proposed, together with labelling and safety rules.
Descriptors (primary) mutagens; laboratories; safe working methods; laboratory work; ethidium bromide
Descriptors (secondary) dangerous substances; DNA; detection by fluorescence; limitation of exposure; exposure evaluation; labelling
Document type D - Periodical articles
Country / State or ProvinceBelgium
Subject(s) Commerce, services, offices
Broad subject area(s) Chemical safety
Browse category(ies) Heterocyclic compounds