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Document ID (ISN)100730
CIS number 02-1798
ISSN - Serial title 0019-8366 - Industrial Health
Year 2002
Convention or series no.
Author(s) Kitamura F., Araki S., Suzuki Y., Yokoyama K., Tanigawa Y., Iwasaki R.
Title Assessment of the mutations of p53 suppressor gene and Ha- and Ki-ras oncogenes in malignant mesothelioma in relation to asbestos exposure: A study of 12 American patients
Bibliographic information Apr. 2002, Vol.40, No.2, p.175-181. Illus. 22 ref.
Abstract To examine whether malignant mesothelioma due to asbestos have genetic alterations in the p53 suppressor gene and in Ha- and Ki-ras oncogenes, point mutations of these genes in paraffin-embedded operative open biopsied samples of the primary tumor of malignant mesothelioma of twelve patients were analysed. The genetic analysis was conducted by the polymerase chain reaction single-strand conformation polymorphism method in all patients and by sequencing analysis of DNA bases in the two patients with suspected gene mutation. The analysis of the p53 suppressor gene showed an amino acid converting mutation of exon 7 in one patient and a polymorphism of exon 6 in another patient. No genetic alteration was found in exons 1 and 2 of Ha- and Ki-ras oncogenes in any of the patients. The results suggest that the effects of asbestos exposure on the p53 suppressor gene and Ha- and Ki-ras oncogenes in malignant mesothelioma are negligible.
Descriptors (primary) mutagenic effects; asbestos; mesothelioma
Descriptors (secondary) DNA; mutagenicity tests; smoking
Document type D - Periodical articles
Country / State or ProvinceJapan; USA
Subject(s) Occupational pathology
Broad subject area(s) Chemical safety
Browse category(ies) Antifertility and prenatal effects
Asbestos
Genetic factors in reaction to exposures