|Document ID (ISN)||100730|
|ISSN - Serial title
||0019-8366 - Industrial Health
|Convention or series no.
||Kitamura F., Araki S., Suzuki Y., Yokoyama K., Tanigawa Y., Iwasaki R.
||Assessment of the mutations of p53 suppressor gene and Ha- and Ki-ras oncogenes in malignant mesothelioma in relation to asbestos exposure: A study of 12 American patients
||Apr. 2002, Vol.40, No.2, p.175-181. Illus. 22 ref.
||To examine whether malignant mesothelioma due to asbestos have genetic alterations in the p53 suppressor gene and in Ha- and Ki-ras oncogenes, point mutations of these genes in paraffin-embedded operative open biopsied samples of the primary tumor of malignant mesothelioma of twelve patients were analysed. The genetic analysis was conducted by the polymerase chain reaction single-strand conformation polymorphism method in all patients and by sequencing analysis of DNA bases in the two patients with suspected gene mutation. The analysis of the p53 suppressor gene showed an amino acid converting mutation of exon 7 in one patient and a polymorphism of exon 6 in another patient. No genetic alteration was found in exons 1 and 2 of Ha- and Ki-ras oncogenes in any of the patients. The results suggest that the effects of asbestos exposure on the p53 suppressor gene and Ha- and Ki-ras oncogenes in malignant mesothelioma are negligible.
||mutagenic effects; asbestos; mesothelioma
||DNA; mutagenicity tests; smoking
||D - Periodical articles
|Country / State or Province||Japan; USA|
|Broad subject area(s)
||Antifertility and prenatal effects
Genetic factors in reaction to exposures