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CISDOC database

Document ID (ISN)100728
CIS number 02-1789
ISSN - Serial title 0019-8366 - Industrial Health
Year 2002
Convention or series no.
Author(s) Yamada H., Koizumi S.
Title DNA microarray analysis of human gene expression induced by a non-lethal dose of cadmium
Bibliographic information Apr. 2002, Vol.40, No.2, p.159-166. 57 ref.
Abstract Cadmium (Cd) is a hazardous heavy metal affecting many cellular functions, but little is known on cellular defense mechanisms. This article describes a study of a Cd-induced gene expression profile. Messenger RNA was prepared from HeLa cells exposed to a non-lethal dose of CdSO4, and analysed by the use of an array consisting of 7075 human cDNAs. Many stress response genes including those coding for metallothioneins and heat shock proteins were observed to be induced by Cd. The cellular metabolism inclined toward the synthesis of cysteine and glutathione after Cd exposure. Anti-oxidant genes also appeared to be induced to protect cell components and to quench reactive oxygen species. Ubiquitin pathway was activated as well probably to degrade proteins which might not be renatured. These data suggest that human cells mobilize every genomic resource to overcome cytotoxicity caused by Cd.
Descriptors (primary) cadmium and compounds; cadmium; cytotoxic effects; DNA
Descriptors (secondary) cadmium sulfate; metallothioneins; description of technique; glutathione; cysteine
Document type D - Periodical articles
Country / State or ProvinceJapan
Subject(s) Toxic and dangerous substances
Broad subject area(s) Chemical safety
Browse category(ies) Cadmium and compounds
Inorganic substances