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Document ID (ISN)109038
CIS number 09-193
ISSN - Serial title 1545-9624 - Journal of Occupational and Environmental Hygiene
Year 2008
Convention or series no.
Author(s) Johnson D.L., Lynch R.A.
Title An efficient analytical method for particle counting in evaluating airborne infectious isolation containment using fluorescent microspheres
Bibliographic information Apr. 2008, Vol.5, No.4, p.271-277. Illus. 8 ref.
Abstract The containment efficiency of patient isolation enclosures in hospitals must be verified to protect staff, other patients and visitors. This article reports on the development and validation of a simple technique to measure the low count concentrations of escaping airborne particles that might be present outside an isolation enclosure, based on micrometer-sized fluorescent polystyrene latex microspheres as a surrogate for pathogenic bioaerosols. Particles are released into the isolation enclosure and air is sampled inside and outside the room to capture airborne particles on 25mm diameter filters, which then allows the number of particles to be quantified using a random field counting approach. The method was found to be specific, sensitive and accurate.
Descriptors (primary) confined spaces; reliability; biological hazards; hospitals; infection control
Descriptors (secondary) aerosols; evaluation of technique; description of technique; determination in air; filtration sampling; number concentration determination
Document type D - Periodical articles
Country / State or ProvinceUSA
Subject(s) Toxic and dangerous substances
Broad subject area(s) Biological hazards
Browse category(ies) Confined spaces
Biological hazards
Health care services