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Document ID (ISN)100405
CIS number 02-1451
ISSN - Serial title 0002-8894 - American Industrial Hygiene Association Journal
Year 2002
Convention or series no.
Author(s) Reynolds S.J., Thorne P.S., Donham K.J., Croteau E.A., Kelly K.M., Lewis D., Whitmer M., Heederik D.J.J., Douwes J., Connaughton I., Koch S., Malmberg P., Larsson B.M., Milton D.K.
Title Comparison of endotoxin assays using agricultural dusts
Bibliographic information July-Aug. 2002, Vol.63, No.4, p.430-438. Illus. 46 ref.
Abstract The objective of this study was to compare endotoxin analyses in laboratories using Limulus-based assays for analysis of organic dusts in three agricultural environments: chicken barns, swine barns and corn processing facilities. Precision of assays performed within laboratories was good, with pooled coefficients of variation for replicate samples ranging from 1 to 11% over all laboratories and dust types. There were significant differences among laboratories for all three dust types. The pattern of differences between laboratories varied by dust type. For chicken dust, laboratories using the endpoint method reported higher results than those using kinetic methods. For swine and corn dusts, laboratories using the kinetic method reported the highest endotoxin values. Statistical differences in performance between laboratories may be related to extraction and analysis methods. These findings will be useful for the standardization of sampling and analysis of airborne endotoxin in agriculture.
Descriptors (primary) airborne dust; bacterial toxins; statistical evaluation; test laboratories; organic dust
Descriptors (secondary) livestock rearing; maize; poultry farming; comparative analysis; dust analysis; description of technique; sampling and analysis
Document type D - Periodical articles
Country / State or ProvinceAustralia; Netherlands; Sweden; USA
Subject(s) Toxic and dangerous substances
Broad subject area(s) Biological hazards
Browse category(ies) Statistical methods